• Lee, Man Ryul, Ph.D.

    Professor, Vice Director of SIMS Research Planning Division

    hESC, hiPSC, Metabolic Reprogramming, microRNA, Organoid

    Room 209, SIMS


Laboratory of Stem Cell Regulation (Stemopia)

Our group research interests focus on the early human development and identifying the causes of diseases using human pluripotent stem cell (hESC) and cord blood hematopoietic stem cell (HSC). To understand such complex and diverse biological mechanisms underlying the human’s early development and diseases, we take multi-omics approaches such as transcriptome, metabolome, and single cell transcriptome to examine relations of genotype-phenotype-functionality.


I. Analysis on the Characteristics of Human Pluripotent Stem Cell

As Human pluripotent stem cells are equipped with an ability to self-renew and differentiate into ~200 functional cells, they are a promising source of cell therapeutic products with unique cell functions and mechanism. A core area of research addresses changes in intracellular organelle, intracellular stress reaction, and metabolic mechanism of three types of human pluripotent stem cells; embryonic stem cell (ESC), induced pluripotent stem cell (iPSC), and somatic cell nuclear transfer stem cell (SCNT). Especially, in order to explore changes in intracellular organelles and stress during the process of differentiation/reprogramming, transcriptome, microRNAs, metabolome, and single cell transcriptome are routinely performed to provide evidence to develop highly efficacious cell therapeutic agents.


II. Establishment of Organoid & Disease Modeling

We are currently establishing a protocol for a multi-organoid, which provides an in vitro tool to elucidate the causes of genetic disorders or intractable diseases, and disease modeling. We establish patient-specific iPSCs from the individuals suffering from a genetically inherited disorder and using an hESC line with a genetic defect, we have established a vascular modeling system to study the cause and find a cure for hereditary neurovascular disease. In addition, we perform the functional analysis of lung that has a genetic deficiency at the single cell level using a lung organoid.


III. Development of Metabolism-Targeting New Drug for Acute Leukemia

The incidence rate of acute leukemia is constantly increasing by 5-10% every year, but only ~30% of the patient is cured by the existing medicines. It is necessary to explore the unique and new metabolic mechanisms of cancer cells for the development next-generation anticancer drugs. One strategy would be to develop a targeted anticancer drug that can detect and control the specific metabolism of tumor cells, especially metabolic regulatory enzymes in the mitochondria. In our lab, a joint research team is formed to develop an anti-cancer drug targeting a mitochondria specific carbonic anhydrase 5 (CA5A/B). An in-depth analysis of metabolome and mechanism of leukemic cells would open the way for developing a new metabolism-targeting anti-cancer drug

Principal Investigator

Man Ryul Lee Ph.D.


2002. B.S. in Animal Bioscience and Technology, KonKuk University, Korea

2004. M.S. in Biomedical Sciences & Engineering, Korea University, Korea

2009. Ph.D. in Biomedical Sciences, Hanyang University, Korea

2015. Postdoctoral fellow, Indiana University, USA

2019. Assistant Professor, Soonchunhyang Institute of Medi-bio Science (SIMS)
Associate Professor, Soonchunhyang Institute of Medi-bio Science (SIMS)

Present.   Professor, Soonchunhyang Institute of Medi-bio Science (SIMS)

Research Interest

1. The study of miRNA-mediated pluripotency and somatic cell reprogramming

2. The study of cellular organelles in maintaining stem cell niche

3. The establishment of organoid and disease modeling using iPSC

4. Development of metabolism-targeting new drug for acute leukemia

Graduate Students


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하태원 (Tae Won Ha)

Education: 2017, B.S. in Soonchunhyang University

present MS/Ph.D. in SIMS

Laboratory: Lab. of Stem Cell Regulation


Research Field: Role of exosomal miRNAs in PSCs early differentiation


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김현규 (Hyun Kyu Kim)

Education: 2016, B.S. in Sun Moon University

2018, M.S. in Sun Moon University

present Ph.D. graduate student in SIMS

Laboratory: Lab. of Stem Cell Regulation


Research Field: Mechanism of metabolic conversion and energy homeostasis during reprogramming


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임정숙 (Jeong Suk Im)

Education: 2019, B.S. in Soonchunhyang University

present MS graduate student in SIMS

Laboratory: Lab. of Stem Cell Regulation


Research Field: Effect of tight junction on maintain stemness


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송예나 (Song Yena)

Education: 2016, B.S. in Mokpo National University

present MS graduate student in SIMS

Laboratory: Lab. of Stem Cell Regulation


Research Field: Research on somatic cell reprogramming and lung disease using lung organoid

Byung Hoo Song, Su Young Son, Hyun Kyu Kim, Tae Won Ha, Jeong Suk Im, Aeli Ryu, Hyeji Jeon, Hee Yong Chung, Jae Sang Oh, Choong Hwan Lee and Man Ryul Lee, Profiling of Metabolic Differences Between Hematopoietic Stem Cells and Acute/Chronic Myeloid Leukemia. Metabolites 2020, 10, 427

Ji Hyun Kwon, Hyun Kyu Kim, TaeWon Ha, Jeong Suk Im, Byung Hoo Song, Ki Sung Hong, Jae Sang Oh, Jaeseok Han and Man Ryul Lee, Hyperthermia Disturbs and Delays Spontaneous Differentiation of Human Embryoid Bodies. Biomedicines 2020, 8(6) 176

Tae Won Ha, Ji Hun Jeong, HyeonSeok Shin, Hyun Kyu Kim, Jeong Suk Im, Byung Hoo Song, Jacob Hanna, Jae Sang Oh, Dong-Hun Woo, Jaeseok Han, and Man Ryul Lee, Characterization of Endoplasmic Reticulum (ER) in Human Pluripotent Stem Cells Revealed Increased susceptibility to Cell Death upon ER stress. Cells, 2020, 9, 1078

Jeong Seong Kim, Seon In Hwang, Jung Lim Ryu, Hee Su Hong, Ji-Min Lee, Sang Min Lee, Xiong Jin, Choongseong Han, Jong-Hoon Kim, Jaeseok Han, Man Ryul Lee, Dong-Hun Woo, ER stress reliever enhances functionalities of in vitro cultured hepatocytes. Stem Cell Research, Vol 43, March 2020, 101732

Heung-Mo Yang, Jong-Jin Choi, Ha-Na Kim, Seung Jip Yang, Soon-Jung Park, Changhee Kang, Hyung-Min Chung, Man Ryul Lee, Sung Joo Kim, Sung-Hwan Moon. (2019) Reconstituting human cutaneous regeneration in humanized mice under endothelial cell therapy. Journal of Investigative Dermatology 139(3) 697-701

Bin Guo, Xinxin Huang, Man Ryul Lee, Sang A Lee & Hal E Broxmeyer (2018) Antagonism of PPAR-γ signaling expands human hematopoietic stem and progenitor cells by enhancing glycolysis. Nature Medicine, Mar; 23(3):360-367

Soon-Jung Park, Sang A Lee, Nutan Prasain, Daekyeong Bae, Hyunsu Kang, Taewon Ha, Jong Soo Kim, Ki-Sung Hong, Charlie Mantel, Sung-Hwan Moon, Hal E. Broxmeyer, and Man Ryul Lee (2017) Metabolome profiling of partial and fully reprogrammed iPSCs. Stem Cells and Development, 26, (10) 734-742

Man Ryul Lee, Charlie Mantel, Sang A Lee, Sung-Hwan Moon and Hal E. Broxmeyer. (2016) MiR-31/SDHA axis Regulates Reprogramming Efficiency through Mirtochondrial Metabolism.Stem Cell Reports, 2(7) 1-10

Nutan Prasain, Man Ryul Lee, Sasidhar Vemula, Jonathan Luke Meador, Momoko Yoshimoto, Michael J. Ferkowicz, Alexa Fett1, Manav Gupta, Brian M. Rapp, Mohammad Reza Saadatzadeh, Michael Ginsberg, Olivier Elemento, Younghee Lee, Sherry L. Voytik-Harbin, Hyung Min Chung, Ki Sung Hong, Emma Reid, Christina L. O'Neill, Medina J. Reinhold, Alan W. Stitt, Michael P. Murphy, Shahin Rafii, Hal E. Broxmeyer, and Mervin C. Yoder. (2014) Differentiation of human pluripotent stem cells to cells similar to cord-blood endothelial colony-forming cells. Nature Biotechnology Vol.32,(11)1151-1157