순천향의생명연구원(SIMS)

STEP1
Education

· Complete SCH IACUC Ethics education

· SIMS Training for utilizing Animal Facility (1time/month, Notice)
IACUC
STEP2
Education

1. Before writing an animal testing protocol, you should contact the chairman of the SIMS IACUC to obtain approval.

2. Animal application

3. Apply for animal purchase

4. Delivery date : Tuesday or Thursday
※The delivery date may change depending on holidays and company situations.

5. Apply to carry in animals

1) Submit [Application for carrying in animals] and a monitoring report covering more than 1 year, documented by the breeding institution.

2) After being approved by the SIMS IACUC, bring animals into the quarantine room in the SIMS animal laboratory.

3) Laboratory animals which have been brought into the quarantine room are reared for 4 weeks, and the Korea Research Institute of Bioscience and Biotechnology is requested to perform microbial monitoring (31 items). The cost of the monitoring is paid for by the investigator.
STEP3
Approval
STEP4
Use

· User training based on the SIMS animal laboratory SOP must be observed.
STEP5
Payment

· Usage fee is calculated on a daily basis for each cage, and monthly bills will be sent via e-mail at the beginning of each month.

Usage fee

Item	Area	Soonchunhyang	Others
1 cage/day	SPF	₩ 900	₩ 1,800

※ If additional cage changes and drinking water changes beyond the normal management cycle are required due to the nature of the experiment, additional fees for replacing cages and drinking water will be charged.
※Management cycle
- Cage change: 1 time/week
- Water change: 2 times/week

Items

Mouse	Items (31 items)
	Sendai virus (HVJ)
	Mouse hepatitis virus (MHV)
	Hanta virus(Hemorrhagic fever with renal syndroma)
	Lymphocytic choriomeningitis(LCM)virus
	Mouse encephalomyelitis virus(GDVII)
	Pnemonia virus of mice(PVM)
	Ectromelia virus(agent causing mouse pox)
	K virus
	Lactic dehydrogenase elevating virus (LDEV)
	Mouse parvovirus(MPV)
	Mouse thymic virus(MTV)
	Polyoma virus
	Reovirus 3(REO 3)
	Rotavirus (Epizootic diarrhea of infant mice[EDIM])
	Minute virus of mouse(MVM)
	Mouse adenovirus(MAV)
	Mouse Norovirus(MNV)
	Mycoplasma pulmonis
	Pasteurella pneumotropica
	Salmonella spp.
	Corynebacterium kutscheri
	Pseudomonas aeruginosa
	Clostridium piliforme (Tyzzer's disease)
	Cilia-associated respiratory(CAR) bacillus
	Intestinal protozoa
	Syphacia spp.
	Ectoparasite
	Helicobacter hepaticus
	H.bilis
	Streptococcus
	Staphylococcus aureus

Routine Monitoring: Regular inspection for microbial contamination in breeding animals 4 times per year (quarterly)

Special Monitoring

Quarantine	Microbial testing for animals when they are carried in.
In case of suspected disease outbreak	Mass stranding
	Weaning rate for the living falls suddenly
	A large number of animals with a bent back appear
	Sudden decrease in feed intake
	Tremors and abnormal behavior are observed in animals
	A large number of animals avoid people (anxiety) etc.

1) Submit [Application for carrying in animals] and a monitoring report covering more than 1 year, documented by the breeding institution.

2) After being approved by the SIMS IACUC, bring animals into the quarantine room in the SIMS animal laboratory.

3) Laboratory animals which have been brought into the quarantine room are reared for 4 weeks, and the Korea Research Institute of Bioscience and Biotechnology is requested to perform microbial monitoring (31 items). The cost of the monitoring is paid for by the investigator.